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Pools of pre-developed primers might help generate complete expression profiles, In particular exactly where assay sensitivity is critically vital.
Unlike oligo(dT) primers, these primers will not be template precise and they are well suited for DNA synthesis utilizing Klenow fragments with DNA templates or for cDNA synthesis utilizing reverse transcriptase with mRNA templates.
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Compared with oligo(dT) primers, these primers will not be template precise and therefore are suited to DNA synthesis utilizing Klenow fragments with DNA templates or for cDNA synthesis employing reverse transcriptase with mRNA templates.
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In distinction to random primers, oligo (dT) primers usually are not suitable for degraded samples but are superb for developing cDNA libraries and encouraged for use with reverse transcriptases (RTs) as Superscript IV RT.
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